| Structural highlights
Function
PINI_ARATH Acts as a component of the auxin efflux carrier. Seems to be involved in the basipetal auxin transport. Mediates the formation of auxin gradient which is required to ensure correct organogenesis. Coordinated polar localization of PIN1 is directly regulated by the vesicle trafficking process and apical-basal PIN1 polarity also depends on the phosphorylation of conserved serine residues by PID kinase. The ARF-GEF protein GNOM is required for the correct recycling of PIN1 between the plasma membrane and endosomal compartments.[1] [2] [3]
Publication Abstract from PubMed
Polar auxin transport is unique to plants and coordinates their growth and development(1,2). The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and drive polar auxin transport(3,4); however, their structures and transport mechanisms remain largely unknown. Here, we report three inward-facing conformation structures of Arabidopsis thaliana PIN1: the apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The transmembrane domain of PIN1 shares a conserved NhaA fold(5). In the substrate-bound structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding. NPA competes with IAA for the same site at the intracellular pocket, but with a much higher affinity. These findings inform our understanding of the substrate recognition and transport mechanisms of PINs and set up a framework for future research on directional auxin transport, one of the most crucial processes underlying plant development.
Structural insights into auxin recognition and efflux by Arabidopsis PIN1.,Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y, Tan S, Friml J, Li D, Liu X, Sun L Nature. 2022 Sep;609(7927):611-615. doi: 10.1038/s41586-022-05143-9. Epub 2022 , Aug 2. PMID:35917925[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Benkova E, Michniewicz M, Sauer M, Teichmann T, Seifertova D, Jurgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. PMID:14651850
- ↑ Huang F, Zago MK, Abas L, van Marion A, Galvan-Ampudia CS, Offringa R. Phosphorylation of conserved PIN motifs directs Arabidopsis PIN1 polarity and auxin transport. Plant Cell. 2010 Apr;22(4):1129-42. doi: 10.1105/tpc.109.072678. Epub 2010 Apr, 20. PMID:20407025 doi:http://dx.doi.org/10.1105/tpc.109.072678
- ↑ Ganguly A, Lee SH, Cho M, Lee OR, Yoo H, Cho HT. Differential auxin-transporting activities of PIN-FORMED proteins in Arabidopsis root hair cells. Plant Physiol. 2010 Jul;153(3):1046-61. doi: 10.1104/pp.110.156505. Epub 2010 May, 3. PMID:20439545 doi:http://dx.doi.org/10.1104/pp.110.156505
- ↑ Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y, Tan S, Friml J, Li D, Liu X, Sun L. Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature. 2022 Sep;609(7927):611-615. PMID:35917925 doi:10.1038/s41586-022-05143-9
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