Structural highlights
7yrk is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 0.92Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]
Publication Abstract from PubMed
A novel hybrid protein composed of a superoxide dismutase-active Cu(II) complex (CuST) and lysozyme (CuST@lysozyme) was prepared. The results of the spectroscopic and electrochemical analyses confirmed that CuST binds to lysozyme. We determined the crystal structure of CuST@lysozyme at 0.92 A resolution, which revealed that the His15 imidazole group of lysozyme binds to the Cu(II) center of CuST in the equatorial position. In addition, CuST was fixed in position by the weak axial coordination of the Thr89 hydroxyl group and the hydrogen bond between the guanidinium group of the Arg14 residue and the hydroxyl group of CuST. Furthermore, the combination of CuST with lysozyme did not decrease the superoxide dismutase activity of CuST. Based on the spectral, electrochemical, structural studies, and quantum chemical calculations, an O(2)(-) disproportionation mechanism catalyzed by CuST@lysozyme is proposed.
A novel hybrid protein composed of superoxide-dismutase-active Cu(II) complex and lysozyme.,Furuya T, Nakane D, Kitanishi K, Katsuumi N, Tsaturyan A, Shcherbakov IN, Unno M, Akitsu T Sci Rep. 2023 Apr 27;13(1):6892. doi: 10.1038/s41598-023-33926-1. PMID:37106030[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
- ↑ Furuya T, Nakane D, Kitanishi K, Katsuumi N, Tsaturyan A, Shcherbakov IN, Unno M, Akitsu T. A novel hybrid protein composed of superoxide-dismutase-active Cu(II) complex and lysozyme. Sci Rep. 2023 Apr 27;13(1):6892. PMID:37106030 doi:10.1038/s41598-023-33926-1
