7zjx
From Proteopedia
Rabbit 80S ribosome programmed with SECIS and SBP2
Structural highlights
DiseaseSEBP2_HUMAN Short stature-delayed bone age due to thyroid hormone metabolism deficiency. The disease is caused by variants affecting the gene represented in this entry. FunctionSEBP2_HUMAN Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Publication Abstract from PubMedThe elongation of eukaryotic selenoproteins relies on a poorly understood process of interpreting in-frame UGA stop codons as selenocysteine (Sec). We used cryo-electron microscopy to visualize Sec UGA recoding in mammals. A complex between the noncoding Sec-insertion sequence (SECIS), SECIS-binding protein 2 (SBP2), and 40S ribosomal subunit enables Sec-specific elongation factor eEFSec to deliver Sec. eEFSec and SBP2 do not interact directly but rather deploy their carboxyl-terminal domains to engage with the opposite ends of the SECIS. By using its Lys-rich and carboxyl-terminal segments, the ribosomal protein eS31 simultaneously interacts with Sec-specific transfer RNA (tRNA(Sec)) and SBP2, which further stabilizes the assembly. eEFSec is indiscriminate toward l-serine and facilitates its misincorporation at Sec UGA codons. Our results support a fundamentally distinct mechanism of Sec UGA recoding in eukaryotes from that in bacteria. Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon.,Hilal T, Killam BY, Grozdanovic M, Dobosz-Bartoszek M, Loerke J, Burger J, Mielke T, Copeland PR, Simonovic M, Spahn CMT Science. 2022 Jun 17;376(6599):1338-1343. doi: 10.1126/science.abg3875. Epub 2022, Jun 16. PMID:35709277[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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