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From Proteopedia
TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene
Structural highlights
FunctionTF3A_YEAST Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. Publication Abstract from PubMedRNA Polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here we use cryo-electron microscopy to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Brf1-TBP binding further stabilizes the DNA, resulting in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the mechanism of how the transcription initiation complex assembles on the 5S rRNA promoter, a crucial step in Pol III transcription regulation. Structural basis of TFIIIC-dependent RNA Polymerase III transcription initiation.,Talyzina A, Han Y, Banerjee C, Fishbain S, Reyes A, Vafabakhsh R, He Y bioRxiv. 2023 May 16:2023.05.16.540967. doi: 10.1101/2023.05.16.540967. Preprint. PMID:37292922[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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