8g7p

Publication Abstract from PubMed

The frequency of errors upon decoding of messenger RNA by the bacterial ribosome is low, with one misreading event per 1 x 10(4) codons. In the universal genetic code, the AUN codon box specifies two amino acids, isoleucine and methionine. In bacteria and archaea, decoding specificity of the AUA and AUG codons relies on the wobble avoidance strategy that requires modification of C34 in the anticodon loop of isoleucine transfer RNA(Ile)(CAU) (tRNA(Ile)(CAU)). Bacterial tRNA(Ile)(CAU) with 2-lysylcytidine (lysidine) at the wobble position deciphers AUA while avoiding AUG. Here we report cryo-electron microscopy structures of the Escherichia coli 70S ribosome complexed with elongation factor thermo unstable (EF-Tu) and isoleucine-tRNA(Ile)(LAU) in the process of decoding AUA and AUG. Lysidine in tRNA(Ile)(LAU) excludes AUG by promoting the formation of an unusual Hoogsteen purine-pyrimidine nucleobase geometry at the third position of the codon, weakening the interactions with the mRNA and destabilizing the EF-Tu ternary complex. Our findings elucidate the molecular mechanism by which tRNA(Ile)(LAU) specifically decodes AUA over AUG.

Structures of the ribosome bound to EF-Tu-isoleucine tRNA elucidate the mechanism of AUG avoidance.,Rybak MY, Gagnon MG Nat Struct Mol Biol. 2024 May;31(5):810-816. doi: 10.1038/s41594-024-01236-3. , Epub 2024 Mar 27. PMID:38538914^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.