8gar

From Proteopedia

Nitrosomonas europaea Cytochrome P460 Arg44Ala

Structural highlights

8gar is a 1 chain structure with sequence from Nitrosomonas europaea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.55Å
Ligands:	ACT, HEC
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q50927_NITER

Publication Abstract from PubMed

Cytochrome P460s are heme enzymes that oxidize hydroxylamine to nitrous oxide. They bear specialized "heme P460" cofactors that are cross-linked to their host polypeptides by a post-translationally modified lysine residue. Wild-type N. europaea cytochrome P460 may be isolated as a cross-link-deficient proenzyme following anaerobic overexpression in E. coli. When treated with peroxide, this proenzyme undergoes maturation to active enzyme with spectroscopic and catalytic properties that match wild-type cyt P460. This maturation reactivity requires no chaperones horizontal line it is intrinsic to the protein. This behavior extends to the broader cytochrome c'(beta) superfamily. Accumulated data reveal key contributions from the secondary coordination sphere that enable selective, complete maturation. Spectroscopic data support the intermediacy of a ferryl species along the maturation pathway.

Cytochrome P460 Cofactor Maturation Proceeds via Peroxide-Dependent Post-translational Modification.,Bollmeyer MM, Coleman RE, Majer SH, Ferrao SD, Lancaster KM J Am Chem Soc. 2023 Jul 5;145(26):14404-14416. doi: 10.1021/jacs.3c03608. Epub , 2023 Jun 20. PMID:37338957^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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Outer coordination sphere influences on cofactor maturation and substrate oxidation by cytochrome P460.

Bollmeyer et al. (2023)

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References

↑ Bollmeyer MM, Coleman RE, Majer SH, Ferrao SD, Lancaster KM. Cytochrome P460 Cofactor Maturation Proceeds via Peroxide-Dependent Post-translational Modification. J Am Chem Soc. 2023 Jun 20. PMID:37338957 doi:10.1021/jacs.3c03608

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

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PDB ID 8gar

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Categories: Large Structures | Nitrosomonas europaea | Bollmeyer MM | Lancaster KM

8gar

From Proteopedia

Nitrosomonas europaea Cytochrome P460 Arg44Ala

Structural highlights

Function

Publication Abstract from PubMed

References

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