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8k3r

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S. cerevisiae Chs1 in apo state incubated with GlcNAc

Structural highlights

8k3r is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.51Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHS1_YEAST Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer (PubMed:2941152). Required for mitotic division septum formation during adverse conditions (PubMed:2523889).^[1] ^[2]

Publication Abstract from PubMed

Chitin is one of the most abundant natural biopolymers and serves as a critical structural component of extracellular matrices, including fungal cell walls and insect exoskeletons. As a linear polymer of beta-(1,4)-linked N-acetylglucosamine, chitin is synthesized by chitin synthases, which are recognized as targets for antifungal and anti-insect drugs. In this study, we determine seven different cryo-electron microscopy structures of a Saccharomyces cerevisiae chitin synthase in the absence and presence of glycosyl donor, acceptor, product, or peptidyl nucleoside inhibitors. Combined with functional analyses, these structures show how the donor and acceptor substrates bind in the active site, how substrate hydrolysis drives self-priming, how a chitin-conducting transmembrane channel opens, and how peptidyl nucleoside inhibitors inhibit chitin synthase. Our work provides a structural basis for understanding the function and inhibition of chitin synthase.

Structure, catalysis, chitin transport, and selective inhibition of chitin synthase.,Chen DD, Wang ZB, Wang LX, Zhao P, Yun CH, Bai L Nat Commun. 2023 Aug 8;14(1):4776. doi: 10.1038/s41467-023-40479-4. PMID:37553334^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cabib E, Sburlati A, Bowers B, Silverman SJ. Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J Cell Biol. 1989 May;108(5):1665-72. PMID:2523889 doi:10.1083/jcb.108.5.1665
↑ Bulawa CE, Slater M, Cabib E, Au-Young J, Sburlati A, Adair WL Jr, Robbins PW. The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell. 1986 Jul 18;46(2):213-25. PMID:2941152 doi:10.1016/0092-8674(86)90738-5
↑ Chen DD, Wang ZB, Wang LX, Zhao P, Yun CH, Bai L. Structure, catalysis, chitin transport, and selective inhibition of chitin synthase. Nat Commun. 2023 Aug 8;14(1):4776. PMID:37553334 doi:10.1038/s41467-023-40479-4