8pop

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HK97 small terminase in complex with DNA

Structural highlights

8pop is a 11 chain structure with sequence from Escherichia phage HK97 and Escherichia virus HK97. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9MBW4_BPHK7

Publication Abstract from PubMed

DNA recognition is critical for assembly of double-stranded DNA viruses, in particular for the initiation of packaging the viral genome into the capsid. DNA packaging has been extensively studied for three archetypal bacteriophage systems: cos, pac and phi29. We identified the minimal site within the cos region of bacteriophage HK97 specifically recognised by the small terminase and determined a cryoEM structure for the small terminase:DNA complex. This nonameric circular protein utilizes a previously unknown mechanism of DNA binding. While DNA threads through the central tunnel, unexpectedly, DNA-recognition is generated at its exit by a substructure formed by the N- and C-terminal segments of two adjacent protomers of the terminase which are unstructured in the absence of DNA. Such interaction ensures continuous engagement of the small terminase with DNA, allowing sliding along DNA while simultaneously checking the DNA sequence. This mechanism allows locating and instigating packaging initiation and termination precisely at the cos site.

Structure of HK97 small terminase:DNA complex unveils a novel DNA binding mechanism by a circular protein.,Chechik M, Greive SJ, Antson AA, Jenkins HT bioRxiv [Preprint]. 2023 Jul 20:2023.07.17.549218. doi: , 10.1101/2023.07.17.549218. PMID:37503206[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Chechik M, Greive SJ, Antson AA, Jenkins HT. Structure of HK97 small terminase:DNA complex unveils a novel DNA binding mechanism by a circular protein. bioRxiv. 2023 Jul 20:2023.07.17.549218. PMID:37503206 doi:10.1101/2023.07.17.549218

Contents


PDB ID 8pop

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