8wba

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Cryo-EM structure of the ABCG25 bound to CHS

Structural highlights

8wba is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.1Å
Ligands:Y01
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AB25G_ARATH High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway (PubMed:20133881, PubMed:20935463, PubMed:24521878, PubMed:26517905). Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth (PubMed:26334616).[1] [2] [3] [4] [5]

Publication Abstract from PubMed

Cellular hormone homeostasis is essential for precise spatial and temporal signaling responses and plant fitness. Abscisic acid (ABA) plays pivotal roles in orchestrating various developmental and stress responses and confers fitness benefits over ecological and evolutionary timescales in terrestrial plants. Cellular ABA level is regulated by complex processes, including biosynthesis, catabolism, and transport. AtABCG25 is the first ABA exporter identified through genetic screening and affects diverse ABA responses. Resolving the structural basis of ABA export by ABCG25 is critical for further manipulations of ABA homeostasis and plant fitness. We used cryo-electron microscopy to elucidate the structural dynamics of AtABCG25 and successfully characterized different states, including apo AtABCG25, ABA-bound AtABCG25, and ATP-bound AtABCG25 (E232Q). Notably, AtABCG25 forms a homodimer that features a deep, slit-like cavity in the transmembrane domain, and we precisely characterized the critical residues in the cavity where ABA binds. ATP binding triggers closure of the nucleotide-binding domains and conformational transitions in the transmembrane domains. We show that AtABCG25 belongs to a conserved ABCG subfamily that originated during the evolution of angiosperms. This subfamily neofunctionalized to regulate seed germination via the endosperm, in concert with the evolution of this angiosperm-specific, embryo-nourishing tissue. Collectively, these findings provide valuable insights into the intricate substrate recognition and transport mechanisms of the ABA exporter AtABCG25, paving the way for genetic manipulation of ABA homeostasis and plant fitness.

Structural insights into AtABCG25, an angiosperm-specific abscisic acid exporter.,Xin J, Zhou Y, Qiu Y, Geng H, Wang Y, Song Y, Liang J, Yan K Plant Commun. 2024 Jan 8;5(1):100776. doi: 10.1016/j.xplc.2023.100776. Epub 2023 , Dec 3. PMID:38050355[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. ↑ Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K. ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2361-6. PMID:20133881 doi:10.1073/pnas.0912516107
  2. ↑ Kuromori T, Sugimoto E, Shinozaki K. Intertissue signal transfer of abscisic acid from vascular cells to guard cells. Plant Physiol. 2014 Apr;164(4):1587-92. PMID:24521878 doi:10.1104/pp.114.235556
  3. ↑ Kang J, Yim S, Choi H, Kim A, Lee KP, Lopez-Molina L, Martinoia E, Lee Y. Abscisic acid transporters cooperate to control seed germination. Nat Commun. 2015 Sep 3;6:8113. PMID:26334616 doi:10.1038/ncomms9113
  4. ↑ Kuromori T, Shinozaki K. ABA transport factors found in Arabidopsis ABC transporters. Plant Signal Behav. 2010 Sep;5(9):1124-6. PMID:20935463 doi:10.4161/psb.5.9.12566
  5. ↑ Borghi L, Kang J, Ko D, Lee Y, Martinoia E. The role of ABCG-type ABC transporters in phytohormone transport. Biochem Soc Trans. 2015 Oct;43(5):924-30. PMID:26517905 doi:10.1042/BST20150106
  6. ↑ Xin J, Zhou Y, Qiu Y, Geng H, Wang Y, Song Y, Liang J, Yan K. Structural insights into AtABCG25, an angiosperm-specific abscisic acid exporter. Plant Commun. 2024 Jan 8;5(1):100776. PMID:38050355 doi:10.1016/j.xplc.2023.100776

Contents


PDB ID 8wba

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