Structural highlights
Function
RAD54_YEAST Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed:12453424). Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism (PubMed:32502392). Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized (PubMed:31492866). Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (PubMed:12453424).[1] [2] [3]
References
- ↑ Solinger JA, Kiianitsa K, Heyer WD. Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments. Mol Cell. 2002 Nov;10(5):1175-88. PMID:12453424 doi:10.1016/s1097-2765(02)00743-8
- ↑ Tavares EM, Wright WD, Heyer WD, Le Cam E, Dupaigne P. In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation. Nat Commun. 2019 Sep 6;10(1):4058. PMID:31492866 doi:10.1038/s41467-019-12082-z
- ↑ Crickard JB, Moevus CJ, Kwon Y, Sung P, Greene EC. Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination. Cell. 2020 Jun 11;181(6):1380-1394.e18. PMID:32502392 doi:10.1016/j.cell.2020.04.056
