9v4p
From Proteopedia
Cryo-EM structure of A. thaliana MET1(610-1534) bound covalently to a hemi-mCpG DNA
Structural highlights
FunctionDNMT1_ARATH Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells. Required for MEA promoter methylation in seeds.[1] [2] [3] [4] [5] [6] [7] Publication Abstract from PubMedDNA methylation plays critical roles in eukaryotic gene silencing, genome defense, and the suppression of transposable elements. During DNA replication, DNA methylation is diluted and must therefore be restored through maintenance DNA methylation. In plants, in addition to symmetric CG methylation, non-CG methylation is also abundant, with the maintenance of each DNA methylation pattern employing different pathways. Here, we investigate the molecular basis of CG maintenance methylation by plant METHYLTRANSFERASE 1 (MET1), an ortholog of mammalian DNA Methyltransferase 1 (DNMT1). The cryogenic electron microscopy structure of full-length Arabidopsis (Arabidopsis thaliana) MET1 reveals a unique autoinhibitory mechanism that is distinct from that of DNMT1. The structure of the MET1 catalytic domain in complex with hemimethylated substrate DNA suggests specific recognition of hemimethylated CG DNA and reveals the catalytic mechanism. Overall, our study illuminates the molecular basis of MET1 autoinhibition and its preference for hemimethylated DNA substrates. Structural insights into plant DNA CG methylation maintenance by MET1.,Zhang Z, Li W, Liu Y, Chi C, Nan J, Wang C, Zhu Y, Zhao J, Xue Y, Li Y, Wang P, Zhai J, Du J Plant Cell. 2025 Oct 31;37(11):koaf244. doi: 10.1093/plcell/koaf244. PMID:41082561[8] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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