Catabolite gene activator protein

From Proteopedia

Jump to: navigation, search


Catabolite gene activator protein (CAP) is a DNA-binding protein involved in the transcription of several genes including those involved in the metabolism of sugar. CAP can activate or repress transcription initiation upon binding of cAMP. The cAMP-CAP complex binds to promoters on the DNA. The structure contains a ligand-binding domain (LBD) at the N-terminal and DNA-binding domain (DBD) at the C-terminal. Four cAMP molecules bind to the dimeric CAP. When the amount of glucose transported into the cell is low, cAMP level rises and the cAMP-CAP complex causes increase of transcription of glucose metabolism genes. [1] See also CAMP-dependent pathway and CAMP is second messenger.

1st cAMP binding site

2nd cAMP binding site in E. coli Catabolite gene activator protein (1o3r). [2] Water molecules are shown as red spheres.

3D Structures of catabolite gene activator protein

Catabolite gene activator protein 3D structures


Catabolite gene activator protein complex with cAMP and DNA (1o3r)

Drag the structure with the mouse to rotate

References

  1. ↑ Busby S, Ebright RH. Transcription activation by catabolite activator protein (CAP). J Mol Biol. 1999 Oct 22;293(2):199-213. PMID:10550204 doi:http://dx.doi.org/10.1006/jmbi.1999.3161
  2. ↑ Chen S, Vojtechovsky J, Parkinson GN, Ebright RH, Berman HM. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking. J Mol Biol. 2001 Nov 16;314(1):63-74. PMID:11724532 doi:10.1006/jmbi.2001.5089

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Jaime Prilusky

Retrieved from "http://proteopedia.org/wiki/index.php/Catabolite_gene_activator_protein"
Personal tools