Luciferase

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Function

Luciferase (Luc) or luciferin monooxygenase (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light[1]. See detailed analysis in

See Colored & Bioluminescent Protein.

Relevance

Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success[2]. NanoLuc luciferase is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence [3].

3D Structures of Luciferase

Luciferase 3D structures


Luciferase (PDB code 3iep)

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Contents

Additional information

Luciferase (2d1s) Fancy, high-quality cartoons on/off


Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

References

  1. ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
  2. ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
  3. ↑ England CG, Ehlerding EB, Cai W. NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. Bioconjug Chem. 2016 May 18;27(5):1175-1187. PMID:27045664 doi:10.1021/acs.bioconjchem.6b00112


Additional Literature

  • Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  • Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8

External Resources

Retrieved from "http://proteopedia.org/wiki/index.php/Luciferase"
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