MCherry Fluorescent Protein

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Introduction and Background

mCherry fluorescent protein complex with chromophore and Cl- ions (green) (PDB code 6ylm)

References

Karimi, S., Ahl, D., Vågesjö, E., Holm, L., Phillipson, M., Jonsson, H., & Roos, S. (2016). In Vivo and In Vitro Detection of Luminescent and Fluorescent Lactobacillus reuteri and Application of Red Fluorescent mCherry for Assessing Plasmid Persistence. PloS one, 11(3), e0151969. https://doi.org/10.1371/journal.pone.015196

Laurent, A. D., Mironov, V. A., Chapagain, P. P., Nemukhin, A. V., & Krylov, A. I. (2012). Exploring structural and optical properties of fluorescent proteins by squeezing: modeling high-pressure effects on the mStrawberry and mCherry red fluorescent proteins. The journal of physical chemistry. B, 116(41), 12426–12440. https://doi.org/10.1021/jp3060944

Miyawaki, A., Shcherbakova, D. M., & Verkhusha, V. V. (2012). Red fluorescent proteins: chromophore formation and cellular applications. Current opinion in structural biology, 22(5), 679–688. https://doi.org/10.1016/j.sbi.2012.09.002

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037

Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y., & Remington, S. J. (n.d.). 2H5Q: Crystal structure Of mcherry. Retrieved March 28, 2021, from https://www.ncbi.nlm.nih.gov/Structure/pdb/2H5Q

Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y., & Remington, S. J. (2006). Novel chromophores and buried charges control color in mFruits. Biochemistry, 45(32), 9639–9647. https://doi.org/10.1021/bi060773l

Subach, F. V., Patterson, G. H., Manley, S., Gillette, J. M., Lippincott-Schwartz, J., & Verkhusha, V. V. (2009). Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature methods, 6(2), 153–159. https://doi.org/10.1038/nmeth.1298

Wu, B., Chen, Y., & Müller, J. D. (2009). Fluorescence fluctuation spectroscopy of mCherry in living cells. Biophysical journal, 96(6), 2391–2404. https://doi.org/10.1016/j.bpj.2008.12.3902

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