Myosin light chain kinase

From Proteopedia

Function

Myosin light chain kinase (MLCK) phosphorylates the regulatory light chain of myosin at serine residue 19. This phosphorylation enables the myosin crossbridge to bind to the actin filament and allows muscle contraction to begin. Dephosphorylation of the myosin light chain stops muscle contraction^[1].

Disease

A variant of MLCK is associated with severe asthma in African Americans^[2]. Epithelial MLCK is critical for intestinal barrier dysfunction and hence for pathogenesis of diarrheal diseases^[3].

Two monomers of human myosin light chain kinase residues 40-388 complex with pyrimidine derivative, pentaethylene glycol, ethanediol and sulfate ion (PDB entry 2x4f)

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3D structures of MLCK

Updated on 05-November-2019

2x4f – hMLCK residues 40-388 - human
6c6m - hMLCK 3rd Ig-like domain residues 405-507
2yr3 - hMLCK 4th Ig-like domain residues 510-601 – NMR
2cqv – hMLCK 8th Ig-like domain residues 1238-1338 – NMR

References

↑ Stull JT, Tansey MG, Tang DC, Word RA, Kamm KE. Phosphorylation of myosin light chain kinase: a cellular mechanism for Ca2+ desensitization. Mol Cell Biochem. 1993 Nov;127-128:229-37. PMID:7935354
↑ Flores C, Ma SF, Maresso K, Ober C, Garcia JG. A variant of the myosin light chain kinase gene is associated with severe asthma in African Americans. Genet Epidemiol. 2007 May;31(4):296-305. PMID:17266121 doi:http://dx.doi.org/10.1002/gepi.20210
↑ Clayburgh DR, Barrett TA, Tang Y, Meddings JB, Van Eldik LJ, Watterson DM, Clarke LL, Mrsny RJ, Turner JR. Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo. J Clin Invest. 2005 Oct;115(10):2702-15. Epub 2005 Sep 22. PMID:16184195 doi:http://dx.doi.org/10.1172/JCI24970