NADPH dehydrogenase

From Proteopedia

Function NADPH dehydrogenase or old yellow enzyme or NADH:flavin oxidoreductase/NADH oxidase or prostaglandin F2A synthase or chromate reductase (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor[1]. See NAD(P)H. The acceptor is a quinone. OYE is a flavoenzyme with two cofactors: FAD and FMN. NADPH dehydrogenase quinone 1 (NQO1) reduces quinones to hydroquinones. NADPH dehydrogenase quinone 2 (NQO2) catayzes electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain[2]. Structural highlights OYE cofactor FMN interacts with the ligand in active site[3]. Water molecules are shown as red spheres.

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3D Structures of NADPH dehydrogenase

Updated on 15-July-2024

NADPH dehydrogenase

7blf – OYE + FMN - fungus

1oyc, 1oya – yOYE + FMN - yeast

1oyb – yOYE + FMN + p-hydroxybenzaldehyde

3tjl – SsOYE + FMN + malonate - Scheffersomyces stipitis

6s32 – OYE + FMN + benzamidine – Chroococcidiopsis thermalis

6s31 – GsOYE + FMN + benzaldehyde – Galdieria sulphuraria

6s0g, 6s23 – GsOYE + FMN

6agz – OYE + FMN - Pichia

3kru, 3krz – OYE + FMN – Thermoanaerobacter pseudethanolicus

3atz – TcOYE + FMN + benzaldehyde – Trypanosoma cruzi

3aty – TcOYE + FMN

2gou, 2gqa – SoOYE + FMN – Shewanella oneidensis

2gq9 – SoOYE + FMN + benzaldehyde

7o0t – OYE + FMN – Chloroflexus aggregans

3hgj – TsOYE + FMN + benzaldehyde – Thermus scotoductus

3hf3 – TsOYE + FMN

NADPH dehydrogenase 1

4rnu, 4rnw, 4rnx – yOYE 1 + FMN

1k02, 4h6k, 4k7y – yOYE 1 (mutant) + FMN

1bwk, 1bwl – OYE 1 (mutant) + FMN - Candida albicans

3gr7, 3gr8 – OYE + FMN – Geobacillus kaustophilus

4rnv, 5v4p – yOYE 1 + FMN + p-hydroxybenzaldehyde

1k03 – yOYE 1 (mutant) + FMN + p-hydroxybenzaldehyde

3rnd – yOYE 1 mutant) + FMN + hydroxymethyl cyclopent-2-en-one

4yil, 4ync – yOYE 1 (mutant) + FMN + acrylate derivative

4gbu, 4gxm – yOYE 1 (mutant) + FMN + methyl propenyl phenol

4ge8, 3txz, 4gwe, 4h4i, 4k7v, 4k8e, 4k8h – yOYE 1 (mutant) + FMN + carvone

3tx9 – yOYE 1 + FMN + hydroxymethyl cyclopent-2-en-one

NADPH dehydrogenase 2.6

4qai – SsOYE 2.6 (mutant) + FMN

4m5p – SsOYE 2.6 + FMN + malonate

3upw – SsOYE 2.6 + FMN + malonate + nicotineamide

4df2 – SsOYE 2.6 + FMN + malonate + chlorophenol

NADPH dehydrogenase 3

5v4p – yOYE 3 + FMN + p-hydroxybenzaldehyde

5v4v – yOYE 3 + FMN + nicotinamide

NADPH dehydrogenase 4

4b5n – SoOYE 4 + FMN

NADPH dehydrogenase quinone 1

5a4k – hNQO1 (mutant) + FAD - human

5fuq, 4cet – hNQO1 (mutant) + FAD + dicoumarol

4cf6 – hNQO1 (mutant) + FAD + cibacron blue

References

1. ↑ Ryu JY, Suh KH, Chung YH, Park YM, Chow WS, Park YI. NADPH dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light. Mol Cells. 2003 Apr 30;15(2):240-4. PMID:12803488
2. ↑ Feng Y, Li W, Li J, Wang J, Ge J, Xu D, Liu Y, Wu K, Zeng Q, Wu JW, Tian C, Zhou B, Yang M. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature. 2012 Nov 15;491(7424):478-82. doi: 10.1038/nature11541. Epub 2012 Oct 21. PMID:23086143 doi:http://dx.doi.org/10.1038/nature11541
3. ↑ Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V. The role of glutamine 114 in old yellow enzyme. J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181 doi:10.1074/jbc.M108453200