O-GlcNAc transferase

From Proteopedia

Function O-GlcNac transferase or O-glycosyltransferase (OGT) catalyzes the addition of N-acetylglucosamine (GlcNAc) to serine or threonine residues by O-glycosylation[1]. OGT contains 2 subunits: a 110 kDa and a 78kDa. The 110 kDa subunit contains a superhelical domain with 13 tetratricopeptide (TPR) repeats. The TPR motif consists of 34 amino acids and mediates protein-protein interactions. MurG:UDP-GlcNac is an OGT which forms glycosidic linkage between N-acetyl muramyl pentapeptide and N-acetyl glycosamine in the synthesis of bacterial cell wall[2]. For details see Human O-GlcNAc transferase. Structural highlights OGT active site residues involved in the addition reaction[3]. Water molecule is shown as red sphere. 3D structures of O-GlcNAc transferase O-GlcNAc transferase 3D structures

Jmol._Canvas2D (Jmol) "jmolApplet1"[x] loadScript /wiki/extensions/jsmol/j2s/core/package.js loadScript /wiki/extensions/jsmol/j2s/core/corejmol.z.js loadScript /wiki/extensions/jsmol/j2s/J/translation/PO.js loadScript /wiki/extensions/jsmol/j2s/core/corescript.z.js JSmol exec jmolApplet1 start applet null Jmol JavaScript applet jmolApplet1__330271228890344__ initializing Jmol getValue debug null Jmol getValue logLevel null Jmol getValue allowjavascript null AppletRegistry.checkIn(jmolApplet1__330271228890344__) vwrOptions: { "name":"jmolApplet1","applet":true,"documentBase":"https://proteopedia.org/wiki/index.php/O-GlcNAc_transferase","platform":"J.awtjs2d.Platform","fullName":"jmolApplet1__330271228890344__","codePath":"https:/wiki/extensions/jsmol/j2s/","display":"jmolApplet1_canvas2d","loadStructCallback":"manageLoadStructCallback","signedApplet":"true","appletReadyCallback":"Jmol._readyCallback","statusListener":"[J.appletjs.Jmol object]","syncId":"330271228890344","messageCallback":"msgCb","bgcolor":"white" } setting document base to "https://proteopedia.org/wiki/index.php/O-GlcNAc_transferase" (C) 2015 Jmol Development Jmol Version: 14.31.8 2020-09-26 10:04 java.vendor: Java2Script (HTML5) java.version: 2019-06-08 00:17:46 (JSmol/j2s) os.name: Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com) Access: ALL memory: 0.0/0.0 processors available: 1 useCommandThread: false appletId:jmolApplet1 (signed) Jmol getValue emulate null defaults = "Jmol" Jmol getValue boxbgcolor null Jmol getValue bgcolor white backgroundColor = "white" Jmol getValue ANIMFRAMECallback null Jmol getValue APPLETREADYCallback Jmol._readyCallback APPLETREADYCallback = "Jmol._readyCallback" StatusManager callback set for APPLETREADYCallback f=Jmol._readyCallback cb=APPLETREADY Jmol getValue ATOMMOVEDCallback null Jmol getValue AUDIOCallback null Jmol getValue CLICKCallback null Jmol getValue DRAGDROPCallback null Jmol getValue ECHOCallback null Jmol getValue ERRORCallback null Jmol getValue EVALCallback null Jmol getValue HOVERCallback null Jmol getValue IMAGECallback null Jmol getValue LOADSTRUCTCallback manageLoadStructCallback LOADSTRUCTCallback = "manageLoadStructCallback" StatusManager callback set for LOADSTRUCTCallback f=manageLoadStructCallback cb=LOADSTRUCT Jmol getValue MEASURECallback null Jmol getValue MESSAGECallback msgCb MESSAGECallback = "msgCb" StatusManager callback set for MESSAGECallback f=msgCb cb=MESSAGE Jmol getValue MINIMIZATIONCallback null Jmol getValue SERVICECallback null Jmol getValue PICKCallback null Jmol getValue RESIZECallback null Jmol getValue SCRIPTCallback null Jmol getValue SYNCCallback null Jmol getValue STRUCTUREMODIFIEDCallback null Jmol getValue doTranslate null language=en_US Jmol getValue doTranslate null Note -- Presence of message callback disables disable translation; to enable message translation use jmolSetTranslation(true) prior to jmolApplet() Note -- language translation disabled Jmol getValue popupMenu null Jmol getValue script null Jmol getValue loadInline null Jmol getValue load null Jmol applet jmolApplet1__330271228890344__ ready script 1 started pdbGetHeader = true FileManager opening url https://proteopedia.org/wiki/extensions/Proteopedia/spt/functions.spt?1733933141 loadScript /wiki/extensions/jsmol/j2s/core/coretext.z.js Loading, please wait ... platformSpeed = 8 FileManager opening url https://proteopedia.org/wiki/scripts/46/468106/Cv/1.spt loadScript /wiki/extensions/jsmol/j2s/J/thread/MoveToThread.js spinqualitylabelsall models O-GlcNAc transferase complex with UDP-GlcNAc and sulfate (PDB entry 4gz5) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

References

1. ↑ Pathak S, Alonso J, Schimpl M, Rafie K, Blair DE, Borodkin VS, Schuttelkopf AW, Albarbarawi O, van Aalten DM. The active site of O-GlcNAc transferase imposes constraints on substrate sequence. Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3063. PMID:26237509 doi:http://dx.doi.org/10.1038/nsmb.3063
2. ↑ Hu Y, Chen L, Ha S, Gross B, Falcone B, Walker D, Mokhtarzadeh M, Walker S. Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):845-9. Epub 2003 Jan 21. PMID:12538870 doi:10.1073/pnas.0235749100
3. ↑ Lazarus MB, Jiang J, Gloster TM, Zandberg WF, Whitworth GE, Vocadlo DJ, Walker S. Structural snapshots of the reaction coordinate for O-GlcNAc transferase. Nat Chem Biol. 2012 Dec;8(12):966-8. doi: 10.1038/nchembio.1109. Epub 2012 Oct, 28. PMID:23103939 doi:http://dx.doi.org/10.1038/nchembio.1109