Phosphoenolpyruvate carboxykinase

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Function

Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO2. PEPCK is part of the gluconeogenesis pathway[1]. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.

See also: Gluconeogenesis.

Structural highlights

E. coli GTP-driven PEPCK active site is located in a pocket at the enzyme surface[2]. Water molecules are shown as red spheres.

  • Mn coordination site.
  • Na coordination site.

3D Structures of PEPCK

Phosphoenolpyruvate carboxykinase 3D structures


GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry 1khf)

Drag the structure with the mouse to rotate

References

  1. ↑ Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581
  2. ↑ Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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