Peptide N-glycanase

Function

Peptide N-glycanase (PNG) cleaves oligosaccharides from misfolded proteins and prepares them for degradation. PNG cleaves at β-aspartyl glucosamine bond to remove a glycan moiety from glycoprotein substrate^[1]. PNG is present in prokaryotes and eukaryotes. PNG N terminal PUB domain interacts with the AAA ATPase protein p97 which is a central component in the ubiquitin-proteasome system. Peptide N-glycanase F cleaves Asn-GlcNac bond in N-linked glycoproteins and glycopeptides^[2].

Disease

Mutations in human PNG genes were found in patients showing developmental delay, multifocal epilepsy and other multiple symptoms^[3].

Relevance

PNG F is used in studying of glycoproteins.

Structural highlights

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