Phosphoserine aminotransferase

From Proteopedia

Function Phosphoserine aminotransferase (PSAT) catalyzes the reversible conversion of phosphoserine and oxoglutarate to produce glutamate and 3-phosphonooxypyruvate. PSAT is part of the phosphoserine biosynthesis[1]. Pyridoxal phosphate (vitamin B6, PLP) is a cofactor of PAT. Relevance PSAT is over-expressed in colon tumors and increases their chemoresistance. PSAT1 inhibition is being tested as drug treatment[2]. Structural highlights PSAT overall structure shows a small domain and a large one. The active site contains the PLP co-factor bound to lysine side chain[3]. Water molecules are shown as red spheres.

spinqualitylabelsall models Phosphoserine aminotransferase dimer complex with pyridoxal phosphate, triethylene glycol, Hepes, Cl- and Mg+2 ions (PDB entry 1w23) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of phosphoserine aminotransferase

Updated on 29-August-2023

Phosphoserine aminotransferase

8i28 - yPSAT – yeast

3m5u – PSAT – Campylobacter jejuni

3qm2 – PSAT – Salmonella enterica

5yii - EhPSAT – Entamoeba histolytica

Phosphoserine aminotransferase complex with pyridoxal phosphate

3e77 – hPSAT + PLP – human

8a5v – hPSAT1 + PLP derivative

1bt4, 1w3u, 2c0r – PSAT (mutant) + PLP – Bacillus circulans

1bjn – EcPSAT + PLP – Escherichia coli

1w23, 2bhx, 2bi1, 2bi2, 2bi3, 2bi5, 2bi9, 2bia, 2bie, 2big, 4azj, 4azk - PSAT + PLP – Bacillus alcalophilus

3ffr – PSAT + PLP – Cytophaga hutchinsonii

3qbo – PSAT + PLP

2fyf - PSAT + PLP – Mycobacterium tuberculosis

6czz – AtPSAT1 + PLP – Arabidopsis thaliana

6czx, 6czy – AtPSAT1 + PLP derivative

5yb0 - EhPSAT + PLP

5yd2 - EhPSAT (mutant) + PLP

6xdk - PSAT + PLP – Stenotrophomonas maltophilia

5f8v - PSAT + PLP – Trichomonas vaginalis<br />

7t7j - PSAT + PLP – Klebsiella pneuoniae<br />

Phosphoserine aminotransferase ternary complex

8a5w - hPSAT + PLP derivative + phosphoserine

1bjo - EcPSAT + α-methyl-glutamate + PLP

4xk1 - PSAT + PLP + glutamate – Pseudomonas aeruginosa

References

1. ↑ Basurko MJ, Marche M, Darriet M, Cassaigne A. Phosphoserine aminotransferase, the second step-catalyzing enzyme for serine biosynthesis. IUBMB Life. 1999 Nov;48(5):525-9. PMID:10637769 doi:http://dx.doi.org/10.1080/713803557
2. ↑ Vie N, Copois V, Bascoul-Mollevi C, Denis V, Bec N, Robert B, Fraslon C, Conseiller E, Molina F, Larroque C, Martineau P, Del Rio M, Gongora C. Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells. Mol Cancer. 2008 Jan 25;7:14. doi: 10.1186/1476-4598-7-14. PMID:18221502 doi:http://dx.doi.org/10.1186/1476-4598-7-14
3. ↑ Dubnovitsky AP, Kapetaniou EG, Papageorgiou AC. Enzyme adaptation to alkaline pH: atomic resolution (1.08 A) structure of phosphoserine aminotransferase from Bacillus alcalophilus. Protein Sci. 2005 Jan;14(1):97-110. PMID:15608117 doi:14/1/97