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Phosphotriesterase dimer complex with reaction product ethyl phosphate and Co+2 ion (pink), 3cak

Drag the structure with the mouse to rotate


  1. 1.0 1.1 Roodveldt C, Tawfik DS. Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state. Protein Eng Des Sel. 2005 Jan;18(1):51-8. PMID:15790580 doi:
  2. Bigley AN, Mabanglo MF, Harvey SP, Raushel FM. Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR. Biochemistry. 2015 Aug 25. PMID:26274608 doi:
  3. Kim J, Tsai PC, Chen SL, Himo F, Almo SC, Raushel FM. Structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase. Biochemistry. 2008 Sep 9;47(36):9497-504. Epub 2008 Aug 15. PMID:18702530 doi:10.1021/bi800971v
  4. Griffiths AD, Tawfik DS. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J. 2003 Jan 2;22(1):24-35. doi: 10.1093/emboj/cdg014. PMID:12505981 doi:
  5. Jackson CJ, Foo JL, Tokuriki N, Afriat L, Carr PD, Kim HK, Schenk G, Tawfik DS, Ollis DL. Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase. Proc Natl Acad Sci U S A. 2009 Dec 4. PMID:19966226
  6. Goldsmith M, Eckstein S, Ashani Y, Greisen P Jr, Leader H, Sussman JL, Aggarwal N, Ovchinnikov S, Tawfik DS, Baker D, Thiermann H, Worek F. Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro. Arch Toxicol. 2016 Nov;90(11):2711-2724. doi: 10.1007/s00204-015-1626-2. Epub, 2015 Nov 26. PMID:26612364 doi:
  7. Goldsmith M, Aggarwal N, Ashani Y, Jubran H, Greisen PJ, Ovchinnikov S, Leader H, Baker D, Sussman JL, Goldenzweig A, Fleishman SJ, Tawfik DS. Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers. Protein Eng Des Sel. 2017 Apr 1;30(4):333-345. doi: 10.1093/protein/gzx003. PMID:28159998 doi:
  8. Goldsmith M, Tawfik DS. Enzyme engineering: reaching the maximal catalytic efficiency peak. Curr Opin Struct Biol. 2017 Dec;47:140-150. doi: 10.1016/ Epub , 2017 Oct 16. PMID:29035814 doi:
  9. Goldsmith M, Ashani Y. Catalytic bioscavengers as countermeasures against organophosphate nerve agents. Chem Biol Interact. 2018 Aug 25;292:50-64. doi: 10.1016/j.cbi.2018.07.006. Epub, 2018 Jul 7. PMID:29990481 doi:

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