Polymerase III homoenzyme beta subunit

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The of the Polymerase III holoenzyme is one of ten subunits in the Pol III multisubunit complex, which catalyzes the synthesis of both the leading and lagging strands of DNA in E. Coli. While the core Pol III enzyme (consisting of the alpha, sigma and theta subunits) dissociates from the DNA after replicating approximately 12 residues, the holoenzyme complex has a processivity of more than 5,000 residues. The increase in replication processing is due to the beta subunit.

Structure of Polymerase III Holoenzyme beta subunit (PDB entry 2pol)

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About this Structure

2pol is a 2 chain structure with sequence from Escherichia coli. The June 2012 RCSB PDB Molecule of the Month feature on Sliding Clamps by David Goodsell is 10.2210/rcsb_pdb/mom_2012_6. Full crystallographic information is available from OCA.

See Also


1. Kong XP, Onrust R, O'Donnell M, Kuriyan J. Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp. Cell. 1992 May 1;69(3):425-37. PMID:1349852 Indiani C, O'Donnell M. The replication clamp-loading machine at work in the three domains of life. Nat Rev Mol Cell Biol. 2006 Oct;7(10):751-61. Epub 2006 Sep 6.

2. Inhibition of Protein Interactions with the β2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from β2-Binding Proteins†

3. Gene Wijffels,*,‡, Brian P. Dalrymple,‡, Pavel Prosselkov,§, Kritaya Kongsuwan,‡, V. Chandana Epa,‖, Penelope E. Lilley,§, Slobodan Jergic,§, Jens Buchardt,,¶, Susan E. Brown,§, Paul F. Alewood,, Philip A. Jennings,§,∞ and, and Nicholas E. Dixon§ Biochemistry 2004 43 (19), 5661-5671

4. Escherichia coli Processivity Clamp β from DNA Polymerase III Is Dynamic in Solution Jing Fang, John R. Engen, and Penny J. Beuning Biochemistry 2011 50 (26), 5958-5968

5. Voet, Voet, Pratt. Fundamentals of Biochemistry. 3rd. Ediction. John Wiley and Sons Inc. 2008

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