RNA ligase

From Proteopedia

RNA ligase (LigR) is an enzyme which ligates two ribonucleotide chains using ATP and producing AMP, diphosphate and the ligated ribonucleotide chain[1]. RNA ligase RtcB joins 2',3'-cyclic phosphate and 5'-OH ends[2]. See more details in T4 RNA ligase 2 (Rnl2) and Ligases.

spinqualitylabelsall models Se-Met RNA ligase (PDB code 1vgj) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of RNA ligase

Updated on 05-November-2023

RNA ligase

2c5u – T4LigR 1 RNA-binding domain - Enterobacteria phage T4

1s68 - T4LigR 2 N-terminal

1vgj, 1vdx – PhLigR – Pyrococcus horikoshii

8dcg – PhLigR RtcB

2vug – LigR – Pyrococcus abyssi

1xdn – LigR 1 editing – Trypanosoma brucei

1iuh – LigR 2’-5’ – Thermus thermophilus

5cot – aLigR – amoeba

6vt0, 6vt1, 6vt3, 6vt4, 6vt5, 6vtg, 5cov – aLigR (mutant)

RNA ligase complex

7p3b – hLigR RtcB + GMP + Co - human

5tt6 – T4LigR 1 + ATP

2hvr, 2hvs – T4LigR 2 + DNA + nucleotide

2hvq - T4LigR 2 + adenosine derivative

4qak - LigR 2’-5’ + AMP – Escherichia coli

5cou, 6vt6, 6vt8, 6vt9, 6vtb, 6vtd – aLigR (mutant) + ATP

6vte – aLigR (mutant) + AMP

6vtf – aLigR + pyrophosphate

5d1o, 5d1p – LigR ATP-dependent + ATP – Methanothermobacter thermautotrophicus

4isz, 8dc9, 8dca, 8dcb, 8dcd, 8dcf – PhLigR RtcB + GTP + ion

4dwq, 4it0 – PhLigR RtcB + GMP + Mn

4isj, 4dwr – PhLigR RtcB + Mn

7lfq – PhLigR RtcB + DNA

References

1. ↑ Nichols NM, Tabor S, McReynolds LA. RNA ligases. Curr Protoc Mol Biol. 2008 Oct;Chapter 3:Unit3.15. doi:, 10.1002/0471142727.mb0315s84. PMID:18972386 doi:http://dx.doi.org/10.1002/0471142727.mb0315s84
2. ↑ Tanaka N, Meineke B, Shuman S. RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo. J Biol Chem. 2011 Sep 2;286(35):30253-30257. PMID:21757685 doi:10.1074/jbc.C111.274597