Rtp and Tus DNA Binding

From Proteopedia

Replication Termination Proteins

Rtp and Tus These polar fork bocking sites have been found in yeast, pea, frog and human genomes.

RTP

RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured end, first alpha helix , unstructured loop that is equivalent to the first beta sheet , helix loop helix structure (-), 2 beta sheets with a connecting loop that makes up the 'wing' structure and an additional long alpha helix involved in dimerisation .

Assymmetry of RTP in DNA-binding

: Contacts upstream with rtp dimer bound to A-site

: Contacts with phosphate backbone of downstream DNA

Residues binding to DNA

3D structures of replication termination protein

Replication Termination Protein