Selectin

From Proteopedia

Function Selectin or CD62 are cell adhesion molecules which bind sugar moieties and thus are regarded as lectins. The structure of selectin consists of extracellular calcium-dependent lectin domain, an EGF-like domain and a number of concensus repeat units, a transmembranal domain and a intracellular domain[1]. Three types of selectins are found: E-selectin found in endothelial cells L-selectin found in leukocytes P-selectin found in platelets. Relevance Selectins are involved in inflammation processes. The L-selectins on the surface of leukocytes bind to carbohydrates causing the cells to roll along the blood vessel walls, slowing their motion and enabling them to enter the site of infection[2]. E-selectin levels correlate with disease activity in psoriasis patients[3]. L-selectin levels correlate with disease activity in type I diabetes[4]. Structural insight The tetrasaccharide binding interactions to P-selectin are electrostatic[5]. Ca coordination site.

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3D structures of selectin

Updated on 21-January-2020

P-selectin

1fsb – hCD62P EGF domain – human - NMR

1g1q – hCD62P EGF domain

1g1r – hCD62P EGF domain + tetrasaccharide

1g1s – hCD62P EGF domain +P-selectin glycoprotein ligand peptide

4gxb – hCD62P intracellular domain + sorting nexin-17

L-selectin

3cfw – hCD62L EGF domain

5vc1 – hCD62L EGF domain (mutant)

2lgf – hCD62L calmodulin-binding domain + calmodulin

E-selectin

1esl – hCD62E EGF domain

1g1t – hCD62E EGF domain + tetrasaccharide

4c16 – hCD62 EGF+SCR domains + antagonist

4csy, 6eyi, 6eyj, 6eyk - hCD62 EGF+SCR domains + ligand

References

1. ↑ Tedder TF, Steeber DA, Chen A, Engel P. The selectins: vascular adhesion molecules. FASEB J. 1995 Jul;9(10):866-73. PMID:7542213
2. ↑ Ley K. The role of selectins in inflammation and disease. Trends Mol Med. 2003 Jun;9(6):263-8. PMID:12829015
3. ↑ Szepietowski J, Wasik F, Bielicka E, Nockowski P, Noworolska A. Soluble E-selectin serum levels correlate with disease activity in psoriatic patients. Clin Exp Dermatol. 1999 Jan;24(1):33-6. PMID:10233646
4. ↑ Kretowski A, Gillespie KM, Bingley PJ, Kinalska I. Soluble L-selectin levels in type I diabetes mellitus: a surrogate marker for disease activity? Immunology. 2000 Feb;99(2):320-5. PMID:10692053
5. ↑ Somers WS, Tang J, Shaw GD, Camphausen RT. Insights into the molecular basis of leukocyte tethering and rolling revealed by structures of P- and E-selectin bound to SLe(X) and PSGL-1. Cell. 2000 Oct 27;103(3):467-79. PMID:11081633