Talk:Citrate Synthase

From Proteopedia

Morph details

See 1cts to 2cts (citrate synthase) morph methods.

Ideas for improvement

This "figure-review" was written by Meagan Llewellyn as part of an assignment for a Biochemistry course at Westfield State University, and posted here by the instructor with permission.

1. My favorite figure: My favorite figure is “a morph between the "open" and "closed" states” This shows the two conformations of citrate synthase and how they move from one to the other. There is a PBD code for both the open and closed conformations. Open is PDB code 1cts and closed is PDB code 2cts, where this figure is a mix of the two of these. The script is /wiki/scripts/User:Wayne_Decatur/1cts_to_2cts_(citrate_synthase)_morph_methods/Camorph/5.spt. The primary citation was written in 1982 and is titled Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution, which can be found at https://www.ncbi.nlm.nih.gov/pubmed/?term=7120407.
2. This is my suggestion for a figure legend: The two conformations of citrate synthase. The open conformation shows it in its free enzyme state. Once oxaloacetate binds, the smaller domain undergoes an 18 degree rotation, which results in the closed conformation.
3. What I like about the figure: I like this figure because the animation clearly shows the dramatic conformational change. It makes it easier to understand how this enzyme functions and how the substrates are able to bind.
4. Corresponding figure in the primary citation: I could not find the corresponding figure in the primary citation. Since this is an animation it is based on the two figures already made of the closed and open conformations, as this just shows the rotation that is happening. Both PDB 1cts and 2cts use the same primary citation but there is no corresponding figure to this.
5. How I think the figure could be improved: I think this figure could be improved by labeling the small and large domains. Even if they were just made a different color it would be easier to understand the rotation that is happening because it would be more clear as to what part is moving.

Reference: Remington S, Wiegand G, Huber R. Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution. J Mol Biol. 1982 Jun 15;158(1):111-52. PMID:7120407

--Karsten Theis 20:26, 20 December 2018 (UTC)