Function
Thymidylate kinase (TMK) catalyzes the conversion of thymidine-5’-phosphate (dTMP) and ATP to thymidine-5’-diphosphate and ADP. TMK is important for DNA synthesis[1]. Inhibitors of TMK include bisubstrate compounds of the type TP(n)X where P(n) are the number of phosphoryl groups and X is the nucleoside moiety of the acceptor. For more details see Student Project 8 for UMass Chemistry 423 Spring 2015.
Relevance
The TMK inhibitor azido-dTMP (AZTP) is an HIV AZT prodrug metabolite[2]. Inhibitors of TMK are used as antibacterial agents[3].
Structural highlights
The active site of TMK contains HIV prodrug azido-thymidine, ADP and Mg+2 ion[4].
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3D Structures of thymidylate kinase
Thymidylate kinase 3D structures