DAHP synthase

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<StructureSection load='1kfl' size='350' side='right' caption='E. coli DAHP synthase complex with PEP, phenylalanine, sulfate and Mn+2 ion (PDB code [[1kfl]])' scene='70/708806/Cv/1'>
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<StructureSection load='' size='350' side='right' caption='E. coli DAHP synthase complex with PEP, phenylalanine, sulfate and Mn+2 ion (PDB code [[1kfl]])' scene='70/708806/Cv/1'>
== Function ==
== Function ==
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'''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. DAHPS is a tetramer. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref>
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'''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' or '''phospho-2-dehydro-3-deoxyheptonate aldolase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. <scene name='70/708806/Cv/6'>DAHPS is a tetramer</scene>. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref>
== Structural highlights ==
== Structural highlights ==
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The bivalent metal is bound to a Cys-X-X-His motif. The DAHPS active site is located in a channel at the C-terminal of the enzyme where the substrate (PEP), inhibitor (phenylalanine) and the metal ion (Mn+2) are seen.<ref>PMID:12126632</ref>
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The DAHPS active site is located in a channel at the C-terminal of the enzyme where the <scene name='70/708806/Cv/7'>substrate (PEP)</scene>, <scene name='70/708806/Cv/8'>inhibitor (phenylalanine)</scene> and <scene name='70/708806/Cv/9'>metal ion (Mn+2)</scene> are seen. The bivalent metal is bound to a Cys-X-X-His motif.<ref>PMID:12126632</ref>
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</StructureSection>
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== 3D Structures of DAHP synthase ==
== 3D Structures of DAHP synthase ==
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[[DAHP synthase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*DAHP synthase
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**[[1vr6]] - TmDAHPS – ''Thermotoga maritima''<br />
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**[[3pg8]] - TmDAHPS catalytic domain<br />
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**[[1ofp]] - yDAHPS – yeast<br />
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**[[1ofb]], [[1ofq]] - yDAHPS + Mn<br />
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**[[4uma]], [[4umb]], [[4umc]] - NmDAHPS + Mn – ''Nisseria meningitidis''<br />
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**[[4ixx]] - NmDAHPS (mutant) + Mn <br />
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**[[3tqk]] - DAHPS + Mn – ''Francisella tularensis''<br />
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*DAHP synthase binary complex
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**[[4hsn]] - NmDAHPS + Mn + PEP <br />
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**[[4c1k]] - PfDAHPS + Cd + PEP – ''Pyrococcus furiosus''<br />
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**[[1zco]] - PfDAHPS + Mn + PEP <br />
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**[[1of6]] - yDAHPS + Mn + tyrosine <br />
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**[[1hfb]] - yDAHPS + PEP <br />
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**[[1oab]] - yDAHPS + Mn + PEP <br />
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**[[1ofa]] - yDAHPS + Co + PEP <br />
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**[[1ofr]] - yDAHPS + Mn + phenylalanine<br />
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**[[1og0]] - yDAHPS (mutant) + Mn + phenylalanine <br />
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**[[2b7o]] - MtDAHPS + Mn + PEP – ''Mycobacterium tuberculosis''<br />
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**[[3nud]] - MtDAHPS + phenylalanine<br />
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**[[2ypo]] - MtDAHPS + Mn + phenylalanine<br />
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**[[3nue]] - MtDAHPS + Mn + tryptophan<br />
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**[[2ypp]] - MtDAHPS + Mn + tyrosine<br />
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**[[3nv8]] - MtDAHPS + Mn + PEP <br />
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**[[3pfp]] - MtDAHPS + Mn + inhibitor <br />
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**[[1qr7]] - EcDAHPS + Pb + PEP – ''Escherichia coli''<br />
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**[[1gg1]] - EcDAHPS + Mn + phosphoglycolate <br />
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**[[1n8f]] – EcDAHPS (mutant) + Mn + PEP<br />
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**[[1ofo]] - yDAHPS + phosphoglycolate
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**[[1vs1]] - DAHPS + Mn + PEP – ''Aeropyrum pernix''<br />
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**[[2w19]] - MtDAHPS + chorismate mutase <br />
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*DAHP synthase ternary complex
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**[[1of8]] - yDAHPS + Co + PEP + glycerol-3-phosphate<br />
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**[[1rzm]] - TmDAHPS + Cd + PEP + erythrose-4-phosphate<br />
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**[[3pg9]] – TmDAHPS + tyrosine + inhibitor<br />
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**[[1kfl]] - EcDAHPS + Mn + PEP + phenylalanine <br />
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**[[4hso]] - MnDAHPS (mutant) + Mn + PEP + tyrosine <br />
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**[[2w1a]] - MtDAHPS + Mn + TSA + chorismate mutase <br />
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**[[3kgf]], [[3rzi]] - MtDAHPS + Mn + phenylalanine + tryptophan<br />
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**[[2ypq]] - MtDAHPS + Mn + tyrosine + tryptophan<br />
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}}
 
== References ==
== References ==
<references/>
<references/>
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[[Category:Topic Page]]

Current revision

E. coli DAHP synthase complex with PEP, phenylalanine, sulfate and Mn+2 ion (PDB code 1kfl)

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References

  1. ↑ Stephens CM, Bauerle R. Analysis of the metal requirement of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. J Biol Chem. 1991 Nov 5;266(31):20810-7. PMID:1682314
  2. ↑ Shumilin IA, Zhao C, Bauerle R, Kretsinger RH. Allosteric inhibition of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase alters the coordination of both substrates. J Mol Biol. 2002 Jul 26;320(5):1147-56. PMID:12126632

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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