2ouc

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[[Image:2ouc.jpg|left|200px]]<br /><applet load="2ouc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ouc, resolution 2.20&Aring;" />
 
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'''Crystal structure of the MAP kinase binding domain of MKP5'''<br />
 
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==Overview==
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==Crystal structure of the MAP kinase binding domain of MKP5==
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MAP kinase phosphatases (MKPs) have crucial roles in regulating the, signaling activity of MAP kinases and are potential targets for drug, discovery against human diseases. These enzymes contain a catalytic domain, (CD) as well as a binding domain (BD) that help recognize the target MAP, kinase. We report here the crystal structures at up to 2.2 A resolution of, the BD and CD of human MKP5 and compare them to the known structures from, other MKPs. Dramatic structural differences are observed between the BD of, MKP5 and that of MKP3 determined previously by NMR. In particular, the, cluster of positively charged residues that is important for MAP kinase, binding is located in completely different positions in the two, structures, with a distance of 25 A between them. Moreover, this cluster, is alpha-helical in MKP5, while it forms a loop followed by a beta-strand, in MKP3. These large structural differences could be associated with the, distinct substrate preferences of these phosphatases, but further studies, are needed to confirm this. The CD of MKP5 is observed in an active, conformation, and two loops in the active site have backbone shifts of up, to 5 A relative to the inactive CDs from other MKPs.
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<StructureSection load='2ouc' size='340' side='right'caption='[[2ouc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ouc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OUC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ouc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ouc OCA], [https://pdbe.org/2ouc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ouc RCSB], [https://www.ebi.ac.uk/pdbsum/2ouc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ouc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DUS10_HUMAN DUS10_HUMAN] Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.<ref>PMID:22375048</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/2ouc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ouc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2OUC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OUC OCA].
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*[[MAP kinase phosphatase|MAP kinase phosphatase]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the MAP kinase binding domain and the catalytic domain of human MKP5., Tao X, Tong L, Protein Sci. 2007 May;16(5):880-6. Epub 2007 Mar 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17400920 17400920]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Tao, X.]]
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[[Category: Tao X]]
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[[Category: Tong, L.]]
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[[Category: Tong L]]
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[[Category: hydrolase]]
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[[Category: rhodanese fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:17:07 2008''
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Current revision

Crystal structure of the MAP kinase binding domain of MKP5

PDB ID 2ouc

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