4dfh

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of cell adhesion molecule nectin-2/CD112 variable domain

Structural highlights

4dfh is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.85Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The nectin and nectin-like molecule (Necl) family includes important cell adhesion molecules (CAMs) characterized by their Ig-like nature. Such CAMs regulate a broad spectrum of cell-cell interactions, including the interaction between NK cells and cytotoxic T lymphocytes (CTLs) and their target cells. CAM members nectin-2 (CD112) and Necl-5 (CD155) are believed to form homodimers (for nectin-2) or heterodimers in their functions for cell adhesion, as well as to interact with immune costimulatory receptor DNAX accessory molecule 1 (DNAM-1) (CD226) to regulate functions of both NK and CTL cells. However, the structural basis of the interactive mode of DNAM-1 with nectin-2 or Necl-5 is not yet understood. In this study, a soluble nectin-2 Ig-like V-set domain (nectin-2v) was successfully prepared and demonstrated to bind to both soluble ectodomain and cell surface-expressed full-length DNAM-1. The 1.85-A crystal structure of nectin-2v displays a perpendicular homodimer arrangement, revealing the homodimer characteristics of the nectin and Necls. Further mutational analysis indicated that disruption of the homodimeric interface of nectin-2v led to a failure of the homodimer formation, as confirmed by crystal structure and biochemical properties of the mutant protein of nectin-2v. Interestingly, the monomer mutant also loses DNAM-1 binding, as evidenced by cell staining with tetramers and surface plasmon resonance assays. The data indicate that interaction with DNAM-1 requires either the homodimerization or engagement of the homodimeric interface of nectin-2v. These results have implications for immune intervention of tumors or autoimmune diseases in the DNAM-1/nectin-2-dependent pathway.

Crystal Structure of Cell Adhesion Molecule Nectin-2/CD112 and Its Binding to Immune Receptor DNAM-1/CD226.,Liu J, Qian X, Chen Z, Xu X, Gao F, Zhang S, Zhang R, Qi J, Gao GF, Yan J J Immunol. 2012 Jun 1;188(11):5511-20. Epub 2012 Apr 30. PMID:22547693^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu J, Qian X, Chen Z, Xu X, Gao F, Zhang S, Zhang R, Qi J, Gao GF, Yan J. Crystal Structure of Cell Adhesion Molecule Nectin-2/CD112 and Its Binding to Immune Receptor DNAM-1/CD226. J Immunol. 2012 Jun 1;188(11):5511-20. Epub 2012 Apr 30. PMID:22547693 doi:10.4049/jimmunol.1200324

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/4dfh"

@@ Line 1: / Line 1: @@
 ==Crystal structure of cell adhesion molecule nectin-2/CD112 variable domain==
-<StructureSection load='4dfh' size='340' side='right' caption='[[4dfh]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='4dfh' size='340' side='right'caption='[[4dfh]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4dfh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DFH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4dfh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DFH FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dfi|4dfi]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HVEB, PRR2, PVRL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfh OCA], [https://pdbe.org/4dfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dfh RCSB], [https://www.ebi.ac.uk/pdbsum/4dfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dfh ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dfh RCSB], [http://www.ebi.ac.uk/pdbsum/4dfh PDBsum]</span></td></tr>
 </table>
-== Function ==
-[[http://www.uniprot.org/uniprot/PVRL2_HUMAN PVRL2_HUMAN]] Probable cell adhesion protein.<ref>PMID:9657005</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 15: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4dfh" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Poliovirus receptor-related protein|Poliovirus receptor-related protein]]
 == References ==
 <references/>
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Chen, Z]]
+[[Category: Large Structures]]
-[[Category: Gao, F]]
+[[Category: Chen Z]]
-[[Category: Gao, G F]]
+[[Category: Gao F]]
-[[Category: Liu, J]]
+[[Category: Gao GF]]
-[[Category: Qi, J]]
+[[Category: Liu J]]
-[[Category: Qian, X]]
+[[Category: Qi J]]
-[[Category: Xu, X]]
+[[Category: Qian X]]
-[[Category: Yan, J]]
+[[Category: Xu X]]
-[[Category: Zhang, R]]
+[[Category: Yan J]]
-[[Category: Zhang, S]]
+[[Category: Zhang R]]
-[[Category: Cell adhesion]]
+[[Category: Zhang S]]
-[[Category: Cell surface]]
-[[Category: Dnam-1]]

4dfh

From Proteopedia

Current revision

Crystal structure of cell adhesion molecule nectin-2/CD112 variable domain

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox