1t2s

From Proteopedia

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

Structural highlights

1t2s is a 2 chain structure with sequence from Drosophila melanogaster. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AGO2_DROME Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.^[1]

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.

Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.,Lingel A, Simon B, Izaurralde E, Sattler M Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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References

↑ Hammond SM, Boettcher S, Caudy AA, Kobayashi R, Hannon GJ. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science. 2001 Aug 10;293(5532):1146-50. PMID:11498593 doi:http://dx.doi.org/10.1126/science.1064023
↑ Lingel A, Simon B, Izaurralde E, Sattler M. Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain. Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196 doi:http://dx.doi.org/10.1038/nsmb777

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

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PDB ID 1t2s

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1t2s

From Proteopedia

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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