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Structural highlights

Function

[SPT4_YEAST] The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. The complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. Structural and functional component of the centromeric and heterochromatic loci linking chromatin structure with kinetochore function and gene silencing.^{[1] [2] [3] [4] [5] [6] [7]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Elongation factor 3D structures

References

1. ↑ Malone EA, Fassler JS, Winston F. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae. Mol Gen Genet. 1993 Mar;237(3):449-59. PMID:8483459
2. ↑ Hartzog GA, Wada T, Handa H, Winston F. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 1998 Feb 1;12(3):357-69. PMID:9450930
3. ↑ Lindstrom DL, Squazzo SL, Muster N, Burckin TA, Wachter KC, Emigh CA, McCleery JA, Yates JR 3rd, Hartzog GA. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol. 2003 Feb;23(4):1368-78. PMID:12556496
4. ↑ Rondon AG, Garcia-Rubio M, Gonzalez-Barrera S, Aguilera A. Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J. 2003 Feb 3;22(3):612-20. PMID:12554661 doi:http://dx.doi.org/10.1093/emboj/cdg047
5. ↑ Crotti LB, Basrai MA. Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J. 2004 Apr 21;23(8):1804-14. Epub 2004 Apr 1. PMID:15057281 doi:http://dx.doi.org/10.1038/sj.emboj.7600161
6. ↑ Mason PB, Struhl K. Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo. Mol Cell. 2005 Mar 18;17(6):831-40. PMID:15780939 doi:http://dx.doi.org/S1097-2765(05)01116-0
7. ↑ Xiao Y, Yang YH, Burckin TA, Shiue L, Hartzog GA, Segal MR. Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing. PLoS Comput Biol. 2005 Sep;1(4):e39. Epub 2005 Sep 16. PMID:16172632 doi:http://dx.doi.org/10.1371/journal.pcbi.0010039