2wvg

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Structural insights into the pre-reaction state of pyruvate decarboxylase from Zymomonas mobilis

Structural highlights

2wvg is a 4 chain structure with sequence from Zymomonas mobilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:F, MG, TPU
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PDC_ZYMMO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Pyruvate decarboxylase (PDC) uses thiamine diphosphate as an essential cofactor to catalyze the formation of acetaldehyde on the pathway of ethanol synthesis. Here we report the crystallographic image of a prereaction intermediate of a bacterial pyruvate decarboxylase prepared by cocrystallizing the enzyme with pyruvate and a stable analogue of the cofactor's activated ylid form. A second crystal structure of PDC in complex with fluoride shows that the ion organizes a water molecule that occludes the pyruvate binding site, accounting for the inhibitory effect of the halide. Also reported is a structure of the cofactor-free apo form, which when compared to the structure of the holo form indicates how thiamine diphosphate organizes the active site pocket of pyruvate decarboxylase to support catalysis. Guided by the structural and enzymatic data, we propose roles for several key residues in the catalytic mechanism.

Structural Insights into the Prereaction State of Pyruvate Decarboxylase from Zymomonas mobilis .,Pei XY, Erixon KM, Luisi BF, Leeper FJ Biochemistry. 2010 Feb 5. PMID:20099870[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
4 reviews cite this structure
Highlights on contemporary recognition and sensing of fluoride anion in solution and in the solid state.
Cametti et al. (2013)
3 more
10 other articles
No citations found

See Also

References

  1. ↑ Pei XY, Erixon KM, Luisi BF, Leeper FJ. Structural Insights into the Prereaction State of Pyruvate Decarboxylase from Zymomonas mobilis . Biochemistry. 2010 Feb 5. PMID:20099870 doi:10.1021/bi901864j

Contents


PDB ID 2wvg

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OCA

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