4uts

From Proteopedia

Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa

Structural highlights

4uts is a 4 chain structure with sequence from Echinophyllia sp. SC22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.03Å
Ligands:	GYC
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5TLG6_9CNID

Publication Abstract from PubMed

The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the bright "on" and dark "off" states via photoisomerization and proton transfer reactions. We report the room temperature crystal structure of the fast switching Met159Thr mutant of Dronpa at 2.0-A resolution in the bright on state. Structural differences with the wild type include shifted backbone positions of strand beta8 containing Thr159 as well as an altered A-C dimer interface involving strands beta7, beta8, beta10, and beta11. The Met159Thr mutation increases the cavity volume for the p-hydroxybenzylidene-imidazolinone chromophore as a result of both the side chain difference and the backbone positional differences.

Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.,Kaucikas M, Fitzpatrick A, Bryan E, Struve A, Henning R, Kosheleva I, Srajer V, Groenhof G, Van Thor JJ Proteins. 2015 Mar;83(3):397-402. doi: 10.1002/prot.24742. Epub 2015 Jan 13. PMID:25524427^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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References

↑ Kaucikas M, Fitzpatrick A, Bryan E, Struve A, Henning R, Kosheleva I, Srajer V, Groenhof G, Van Thor JJ. Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa. Proteins. 2015 Mar;83(3):397-402. doi: 10.1002/prot.24742. Epub 2015 Jan 13. PMID:25524427 doi:http://dx.doi.org/10.1002/prot.24742

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

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PDB ID 4uts

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4uts

From Proteopedia

Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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