4w29

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70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state.

Structural highlights

4w29 is a 20 chain structure with sequence from Thermus thermophilus HB27 and Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 4qs0, 4qs1, 4qs2 and 4qs3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.8Å
Ligands:ACE, FUA, GDP, MG, NMY
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS3_THET2 Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity).

Publication Abstract from PubMed

Coupled translocation of messenger RNA and transfer RNA (tRNA) through the ribosome, a process catalyzed by elongation factor EF-G, is a crucial step in protein synthesis. The crystal structure of a bacterial translocation complex describes the binding states of two tRNAs trapped in mid-translocation. The deacylated P-site tRNA has moved into a partly translocated pe/E chimeric hybrid state. The anticodon stem-loop of the A-site tRNA is captured in transition toward the 30S P site, while its 3' acceptor end contacts both the A and P loops of the 50S subunit, forming an ap/ap chimeric hybrid state. The structure shows how features of ribosomal RNA rearrange to hand off the A-site tRNA to the P site, revealing an active role for ribosomal RNA in the translocation process.

How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation.,Zhou J, Lancaster L, Donohue JP, Noller HF Science. 2014 Sep 5;345(6201):1188-91. doi: 10.1126/science.1255030. PMID:25190797[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
24 reviews cite this structure
'View From A Bridge': A New Perspective on Eukaryotic rRNA Base Modification.
Sharma et al. (2015)
23 more
78 other articles
No citations found

See Also

References

  1. ↑ Zhou J, Lancaster L, Donohue JP, Noller HF. How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation. Science. 2014 Sep 5;345(6201):1188-91. doi: 10.1126/science.1255030. PMID:25190797 doi:http://dx.doi.org/10.1126/science.1255030

Contents


PDB ID 4w29

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