5tw2

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Structure of mouse CD1d with bound alpha-galactosylsphingamide JG168

Structural highlights

5tw2 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:7LP, FUC, NAG, PLM
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CD1D1_MOUSE Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.[1] [2] [3]

Publication Abstract from PubMed

Invariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic alpha-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed alpha-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied alpha-galactosylsphingamides showed compromised antigenic properties.

Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d.,Guillaume J, Wang J, Janssens J, Remesh SG, Risseeuw MDP, Decruy T, Froeyen M, Elewaut D, Zajonc DM, Calenbergh SV Sci Rep. 2017 Jun 27;7(1):4276. doi: 10.1038/s41598-017-04461-7. PMID:28655912[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
Immunometabolic Activation of Invariant Natural Killer T Cells.
Ververs et al. (2018)
0 more
4 other articles
No citations found

See Also

References

  1. ↑ Jayawardena-Wolf J, Benlagha K, Chiu YH, Mehr R, Bendelac A. CD1d endosomal trafficking is independently regulated by an intrinsic CD1d-encoded tyrosine motif and by the invariant chain. Immunity. 2001 Dec;15(6):897-908. PMID:11754812
  2. ↑ Zajonc DM, Maricic I, Wu D, Halder R, Roy K, Wong CH, Kumar V, Wilson IA. Structural basis for CD1d presentation of a sulfatide derived from myelin and its implications for autoimmunity. J Exp Med. 2005 Dec 5;202(11):1517-26. Epub 2005 Nov 28. PMID:16314439 doi:10.1084/jem.20051625
  3. ↑ Zajonc DM, Cantu C 3rd, Mattner J, Zhou D, Savage PB, Bendelac A, Wilson IA, Teyton L. Structure and function of a potent agonist for the semi-invariant natural killer T cell receptor. Nat Immunol. 2005 Aug;6(8):810-8. Epub 2005 Jul 10. PMID:16007091 doi:10.1038/ni1224
  4. ↑ Guillaume J, Wang J, Janssens J, Remesh SG, Risseeuw MDP, Decruy T, Froeyen M, Elewaut D, Zajonc DM, Calenbergh SV. Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d. Sci Rep. 2017 Jun 27;7(1):4276. doi: 10.1038/s41598-017-04461-7. PMID:28655912 doi:http://dx.doi.org/10.1038/s41598-017-04461-7

Contents


PDB ID 5tw2

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