Structural highlights
6sqc is a 2 chain structure with sequence from Escherichia coli K-12 and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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Method: | X-ray diffraction, Resolution 2.28Å |
Ligands: | , , , , |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Intrinsically disordered proteins (IDPs) constitute a large portion of "Dark Proteome" - difficult to characterize or yet to be discovered protein structures. Here we used conformationally constrained alpha-methylated amino acids to bias the conformational ensemble in the free unstructured activation domain of transcriptional coactivator ACTR. Different sites and patterns of substitutions were enabled by chemical protein synthesis and led to distinct populations of alpha-helices. A specific substitution pattern resulted in a substantially higher binding affinity to nuclear coactivator binding domain (NCBD) of CREB-binding protein, a natural binding partner of ACTR. The first X-ray structure of the modified ACTR domain - NCBD complex visualized a unique conformation of ACTR and confirmed that the key alpha-methylated amino acids are localized within alpha-helices in the bound state. This study demonstrates a strategy for characterization of individual conformational states of IDPs.
Conformational editing of intrinsically disordered protein by alpha-methylation.,Bauer V, Schmidtgall B, Gogl G, Dolenc J, Osz J, Nomine Y, Kostmann C, Cousido-Siah A, Mitschler A, Rochel N, Trave G, Kieffer B, Torbeev V Chem Sci. 2020 Nov 4;12(3):1080-1089. doi: 10.1039/d0sc04482b. PMID:34163874[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bauer V, Schmidtgall B, Gogl G, Dolenc J, Osz J, Nomine Y, Kostmann C, Cousido-Siah A, Mitschler A, Rochel N, Trave G, Kieffer B, Torbeev V. Conformational editing of intrinsically disordered protein by alpha-methylation. Chem Sci. 2020 Nov 4;12(3):1080-1089. doi: 10.1039/d0sc04482b. PMID:34163874 doi:http://dx.doi.org/10.1039/d0sc04482b