D-xylose isomerase

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Contents

Function

D-xylose isomerase (DXI) catalyzes the conversion of D-xylose (DX) to D-xylitol. DXI is part of the fructose and mannose metabolism pathways. Sorbitol is a competitive inhibitor of DXI. DXI is a metal-ion activated tetramer.[1]

Relevance

DXI is used commercially in the production of high fructose corn syrup.

Structural highlights

The biological assembly of D-xylose isomerase from Arthrobacter sp is homotetramer. DXI active site contains 2 Mn+2 ions and the ligand binding pocket is composed of aromatic and acidic residues (water molecules shown as red spheres).[2]

3D structures of D-xylose isomerase

D-xylose isomerase 3D structures


Structure of D-xylose isomerase complex with D-xylose and Mn+2 ions (purple) (PDB code 1xld).

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References

  1. MARSHALL RO, KOOI ER. Enzymatic conversion of D-glucose to D-fructose. Science. 1957 Apr 5;125(3249):648-9. PMID:13421660
  2. Collyer CA, Henrick K, Blow DM. Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening followed by a 1,2-hydride shift. J Mol Biol. 1990 Mar 5;212(1):211-35. PMID:2319597

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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