Insertase

From Proteopedia

Insertase (Ins) catalyzes the insertion of proteins into the prokaryotic plasma membrane. Ins interacts with its substrate in a groove-like structure at an ampiphilic protein-lipid interface allowing the transmembrane segments of the substrate to slide into the lipid bilayer^[1].

Structural highlights

E. Coli Ins (YidC) contains 6 transmembrane segments. Two of these segments was found to interact with a helical segments of a protein which is a candidate for insertion into a plasma membrane^[2].

Cryo EM structure of E. coli insertase (cyan) complex with membrane domain of ATP synthase (green) PDB code 4utq.

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3D structures of insertase

Updated on 03-October-2021

5m5h, 3wvf - EcIns YidC - Escherichia coli - Cryo EM
6al2 - EcIns YidC
3bs6 - EcIns YidC periplasmic domain
4utq - EcIns YidC + ATP synthase subunit C - Cryo EM
5mg3 - EcIns YidC + SECY + SECE + SECG + SECD + SECF - Cryo EM
3wo7, 3wo6 - Ins YidC - Bacillus halodurans
4c4v - EcIns BamA reasidues 344-808
6fsu - EcIns BamA reasidues 421-810
5ekq - Ins BamA + BamC + BamD + BamE
4k3b - Ins BamA - Neisseria gonorrhoeae
4k3c - Ins BamA - Haemophilus Ducreyi
5y82, 5y83 - TmIns YidC (mutant) - Thermotoga maritima
6y86 - TmIns YidC

References

↑ Kuhn A, Kiefer D. Membrane protein insertase YidC in bacteria and archaea. Mol Microbiol. 2017 Feb;103(4):590-594. doi: 10.1111/mmi.13586. Epub 2017 Jan 3. PMID:27879020 doi:http://dx.doi.org/10.1111/mmi.13586
↑ Klenner C, Yuan J, Dalbey RE, Kuhn A. The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis. FEBS Lett. 2008 Dec 10;582(29):3967-72. doi: 10.1016/j.febslet.2008.10.044. Epub , 2008 Nov 6. PMID:18996118 doi:http://dx.doi.org/10.1016/j.febslet.2008.10.044