Journal:Acta Cryst D:S2059798320008475
From Proteopedia
Getting the best of ID30B for RT data collection in microchipsJose A. Gavira, Isaac Rodriguez-Ruiz, Sergio Martinez-Rodriguez, Shibom Basu, Sébastien Teychené, Andrew A. McCarthy and Christoph Mueller-Dieckmann [1] Molecular Tour Furthermore, RT diffraction data can reveal motions crucial for catalysis, ligand binding, and allosteric regulation, not always accessible under standard cryogenic data collection. The chips are fabricated by a combination of either OSTEMER-Kapton or OSTEMER-Mylar materials, both produce a sufficiently low scattering background to permit atomic resolution diffraction data collection at room temperature. The proposed system can be easily incorporated into a fully automatized workflow at any synchrotron beamline facilitating the collection of users' data with no-intervention required of the end-user.
 PDB references: RT structure of HEW Lysozyme obtained at 1.13 A resolution from crystal grown in a Kapton microchip 6ybf; RT structure of HEW Lysozyme obtained at 1.12 A resolution from crystal grown in a Mylar microchip 6ybi; RT structure of Glucose Isomerase obtained at 1.06 A resolution from crystal grown in a Kapton microchip 6ybo; RT structure of Glucose Isomerase obtained at 1.20 A resolution from crystal grown in a Mylar microchip 6ybr; RT structure of Thaumatin obtained at 1.14 A resolution from crystal grown in a Mylar microchip 6ybx; RT structure of Thaumatin obtained at 1.35 A resolution from crystal grown in a Kapton microchip 6yc5. References
| |||||||||||
