User:Fadel A. Samatey/FlgA I
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Interactive 3D Complement in Proteopedia
Scientific Reports an online, open access journal: nature.com/srep
Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica.
Hideyuki Matsunami, Young-Ho Yoon, Vladimir Meshcheryakov, Keiichi Namba, and Fadel A. Samatey.
Scientific Reports 6:27399, June 7, 2016: nature.com/articles/srep27399. (DOI: 10.1038/srep27399)
Molecular TourThis Tour is an Interactive 3D Complement to the journal article[1], with which it assumes that you are familiar. Structure (Open Form)FlgA is a periplasmic chaperone required for the assembly of the peptidoglycan-associated flagellar P-ring, a molecular bushing that supports the rotation of the drive shaft rod for the bacterial flagellum. The structure of FlgA of Salmonella enterica serovar Typhimurium (UniProt P40131) was determined to a resolution of 1.95 Å (3tee, restore initial scene). The structure shown represents the full-length mature protein, 1-198, with the N-terminal signal sequence removed. A 21-residue C-terminal His expression tag present in the crystallized protein was partially disordered and is not shown. Residues 44-47 (TGSA) are missing from the model due to disorder. The single-chain structure has three domains (D1, D2, D3). Domains 1 and 2 are connected by a long beta strand (62-79) that extends into beta sheets in both domains, while domains 2 and 3 are connected by a short coil. Comparison: Open vs. Closed FormsA second crystal form revealed a closed conformation of FlgA (3vki, 2.3 Å resolution). Although described as closed, there is actually no contact between D3 and D1-D2. A morph[2] between the two conformations shows that, relative to the open conformation, D3 moves closer to D1-D2, largely by a bend of about 70o at Arg136 (black ball). The carboxy-terminus in D3 moves about 31 Å closer to D1[3]. Non-motile MutantSalmonella strain SJW1446 is non-flagellated due to the deletion of residues Val140 through Gly143 (ΔVKAG) in the FlgA protein[4]. This region is located in the linker connecting the D2 and D3 domains, as can be seen when 140-143 are highlighted as balls. Disulfide-Locked Closed FormIn the closed form, Arg113 and Ser190 were identified as a suitable location for a disulfide bond that would lock the chain in the closed form. Single mutations R113C or S190C retained motility. Wild type FlgA rescued motility in the mutant strain SJW1446, but double mutant R113C+S190C did not, unless the assay was run in a reducing medium with DTT. Comparison with ThermotogaThe structure of FlgA from Thermotoga maritima was reported in 2009 (3frn, 2.0 Å). It includes domains D1, D2, and D3 each structurally similar to the corresponding domains in FlgA from Salmonella, despite the sequence identity being 16%. However Thermotoga FlgA is longer, including an additional 69-residue domain D0 at the N-terminus. Buttons below will not work unless the green link above is clicked first!
Crystal ContactsCrystal contacts are likely to play a role in determining the open and closed conformations in the crystals analyzed here. The asymmetric unit of the closed form contains four chains, interlocked and apparently stabilizing the closed form. Another way is to show the crystal-contact atoms within 4.0 Å of one chain as balls decorating the surface of the central chain.
It can be seen that the crystal contacts are likely to stabilize the conformation in each case. Nevertheless, the two conformations demonstrate conformational flexibility, and the experiments with FlgA disulfide-locked into the closed conformation, compared with the same mutant in a reducing milieu, indicate that this conformational flexibility is crucial for function.
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Notes and References
- ↑ Matsunami H, Yoon YH, Meshcheryakov VA, Namba K, Samatey FA. Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica. Sci Rep. 2016 Jun 7;6:27399. doi: 10.1038/srep27399. PMID:27273476 doi:http://dx.doi.org/10.1038/srep27399
- ↑ Residues 1-198 from the open form 3tee were morphed to residues 1-198 of chain A (lowest average temperature) of the closed form 3vki. Residues 44-47 were deleted from the closed form since they were missing in the open form due to disorder. The two chains were globally structurally aligned using the "magic fit" (sequence-based structural alignment) tool in Deepview. A 24-frame linear interpolation morph was performed by the morph server kindly provided by User:Karsten Theis.
- ↑ In the closed form, the carboxy terminal alpha carbon is 15 Å from D1 (Met43), while in the open form, that distance is 46 Å.
- ↑ Ohnishi K, Ohto Y, Aizawa S, Macnab RM, Iino T. FlgD is a scaffolding protein needed for flagellar hook assembly in Salmonella typhimurium. J Bacteriol. 1994 Apr;176(8):2272-81. PMID:8157595