Talk:RNaseA Nobel Prizes
From Proteopedia
Ideas for improvement
This "figure-review" was written by Thomas Cabbibo as part of an assignment for a Biochemistry course at Westfield State University, and posted by the instructor with permission.
- My favorite figure: Figure “Tyr92-Pro93”. The figure is based on the coordinates 7RSA and the script is available from http://proteopedia.org//wiki/scripts/Sandbox_Reserved_197/Tyr92-pro93/7.spt. The primary citation is from 1988, and available via https://www.rcsb.org/structure/7RSA.
- This is my suggestion for a figure legend: RNaseA (7RSA) has a Proline at position 93 in its native state is found in the cis conformation. The Proline is shown in the Cis conformation in dark blue, with a Tyrosine bound shown in light blue.
- What I like about the figure: It gives a representation of how the cis conformation is involved in the tight, globular structure of the entire protein.
- Corresponding figure in the primary citation: I was unable to access a corresponding figure in the primary citation. This may be due to the fact that most of my references were published before 2000, so the articles may have never been entered into an online data base.
- How I think the figure could be improved: I think that if the figure had the ability to change from Cis to Trans conformation to show the difference in structure and how it relates to the protein folding. This could help visualize how a change in the Cis conformation disrupts the folding kinetics of the protein causing the whole protein structure, if denatured, to fold back to the original shape of the structure at a much slower rate in the Trans conformation. Here is an example of a set of textbook figures showing the two conformations: https://www.ncbi.nlm.nih.gov/books/NBK22364/figure/A317/.
References
Wlodawer, A., Svensson, L.A., Sjolin, L., Gilliland, G.L.(1988). Structure of phosphate-free ribonuclease A refined at 1.26 A. Biochemistry 27: 2705-2717
--Karsten Theis 19:11, 16 December 2018 (UTC)
