User:Tilman Schirmer/Sandbox 201

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PleD

Contents 1 Overview 2 Substrate binding 3 Allosteric product binding site 4 C-di-GMP 5 Primary inhibition site (Ip) 6 Secondary inhibition site (Is) 7 Primary and secondary inhibition sites 8 Two conformations

Overview

Diguanylate cyclase PleD (1w25) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

from Caulobacter crescentus is a response regulator with an unorthodox catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver () domain, a Rec-like () adaptor domain, and a C-terminal domain that confers the catalytic acitvity.

The GGDEF domain is named after the highly conserved (in PleD it is GGEEF) that locates to a β-hairpin.

Substrate binding

Diguanylate cyclase PleD (2v0n) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The motif is part of the as identified in the structure of PleD in complex with . The GGDEFY domain binds only one GTP subsrate molecule. For the reaction to proceed, two GTP loaded GGDEF domains have to align antiparallely. MODEL.

Allosteric product binding site

Allosteric product binding site Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

C-di-GMP

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Primary inhibition site (Ip)

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Secondary inhibition site (Is)

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Primary and secondary inhibition sites

Two conformations

non-activated (1w25) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

activated (BeF3- modified; 2v0n) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image