TRNase activity

From Proteopedia

Some colicins, such as colicin 5, exhibit tRNase activity as their cytotoxic activity to kill the cell they target. This activity is aimed at the anticodon loops of the tRNA molecule, preventing the tRNA from being recognised in the ribosome. This then prevents the cell from producing proteins, and eventually leads to the death of the cell^[1]. This is able to occur with just a single colicin molecule in the cell, following first order kinetics.

spinqualitylabelsall models PDB ID 2djh Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
2djh, resolution 1.90Å ()
Ligands:
Non-Standard Residues:
Related:	2dfx
Structural annotation: Resources: UniProt : P18000
Functional annotation: Biological process: defense response to bacterium cytolysis Molecular function: nuclease activity endonuclease activity hydrolase activity receptor binding
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

tRNase colicins

Colicin E5
Colicin D

References

1. ↑ Ogawa T, Inoue S, Yajima S, Hidaka M, Masaki H. Sequence-specific recognition of colicin E5, a tRNA-targeting ribonuclease. Nucleic Acids Res. 2006;34(21):6065-73. Epub 2006 Sep 8. PMID:16963495 doi:10.1093/nar/gkl629